| BCRJ: | 0291 |
| Cell Line: | C3A [HepG2/C3A, derivative of Hep G2] |
| Tissue: | |
| Organ: | LIVER |
| Cell Type: | EPITHELIAL |
| Morphology: | EPITHELIAL |
| Pathology: | HEPATOCELLULAR CARCINOMA |
| Scientific Name: | HOMO SAPIENS |
| Vulgar Name: | HUMAN |
| Sex: | MALE |
| Miscelaneous Info: | 15 YEARS ADOLESCENT, CAUCASIAN |
| Virus Succeptibility: | |
| Virus Resistance: | |
| Tumor Formation: | NO |
| Products: | alpha-fetoprotein (alpha fetoprotein); albumin; alpha2 macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin); transferrin; alpha1 antichymotrypsin; (alpha-1-antichymotrypsin); haptoglobin; ceruloplasmin; plasminogen; complement (C4); C3 activator; fibrinogen; alpha1 acid glycoprotein (alpha-1 acid glycoprotein); alpha2 HS glycoprotein (alpha-2-HS-glycoprotein); beta lipoprotein (beta-lipoprotein); retinol binding protein (retinol-binding protein) |
| Dependency Isoenzymes: | |
| Culture Medium: | Eagle's Minimum Essential Medium + 10% SFB |
| Consigner: | |
| Reference: | Kelly JH. Permanent human hepatocyte cell line and its use in a liver assist device (LAD). US Patent 5,290,684 dated Mar 1 1994 |
| Additional Info: | C3A is clonal derivative of Hep G2 that was selected for strong contact inhibition of growth, high albumin production, high production of alpha fetoprotein (AFP) and ability to grow in glucose deficient medium. As the cells become confluent, there is a marked reduction in AFP secretion and an increase in albumin secretion. Gluconeogenesis activity is strongly oxgen dependent. The cells have nitrogen metabolizing activity comparable to perfused rat livers. There is no evidence of a Hepatitis B virus genome in this cell line. |
| ATCC: | CRL-10741 |
| Biosafety: | 1 |
