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| BCRJ: | 0256 | | Cell Line: | ZFL [ZF-L] | | Tissue: | | | Organ: | LIVER | | Cell Type: | EPITHELIAL | | Morphology: | EPITHELIAL | | Pathology: | NORMAL | | Scientific Name: | DANIO RERIO (ZEBRAFISH) | | Vulgar Name: | | | Sex: | | | Miscelaneous Info: |
(The ZFL cell line was established in 1992 from a pool of approximately 10 normal adult zebrafish livers. It exhibits some properties characteristic of liver parenchymal cells). | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | | | Dependency Isoenzymes: | | | Culture Medium: | Complete growth medium: Leibovitz's L-15 medium with 2 mM L-glutamine (Vitacell 30-2008), 50%; Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 4 mM L-glutamine (GIBCO 12100), 35%; Ham's F12 with 1 mM L-glutamine (GIBCO 21700), 15% (all without sodium bicarbonate) and supplemented with: 0.15 g/L sodium bicarbonate 15 mM HEPES 0.01 mg/ml insulin (Sigma # I-1882) 50 ng/ml EGF (do not filter) heat-inactivated fetal bovine serum, 5%
Temperature: 28.0C
Atmosphere: air, 100% | | Consigner: | José M. Monserrat, Fundação Universidade Federal
do Rio Grande (FURG) Departamento de Ciências Fisiológicas Fone: 55 53 32336856/
FAX: XX 53 32336848 Rio Grande (RS)BRASIL | | Reference: | 53447: Ghosh C , et al. Derivation and characterization of a zebrafish liver cell line. Cell Biol. Toxicol. 10: 167-176, 1994. PubMed: 7994634
53448: Miranda CL , et al. Regulation of cytochrome P450 expression in a novel liver cell line from zebrafish (Brachydanio rerio). Arch. Biochem. Biophys. 305: 320-327, 1993. PubMed: 8373170
53449: Collodi P , et al. Induction of zebrafish (Brachydanio rerio) P450 in vivo and in cell culture. Xenobiotica 24: 487-493, 1994. PubMed: 7975714 | | Additional Info: | The cells synthesize and release several proteins into the culture medium, including a 70 kDa protein recognized by anti-bovine serum albumin IgG. [53447]
ZFL cell homogenates exhibit alanine and aspartate aminotransferase, glucose-6-phosphatase and alkaline phosphatase enzyme activities. [53447]
The dioxin, TCDD, induces one or possibly two forms of cytochrome P450 immunochemically and functionally related to rainbow trout P4501A1. [53448]
The cells are useful for studies of liver cell metabolism and xenobiotic formation. They may be used for in vitro toxicology studies. | | ATCC: | CRL-2643 | | Biosafety: | 01 |
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