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BCRJ:	0254
Cell Line:	Y-1
Tissue:
Organ:	ADRENAL CORTEX
Cell Type:	B LYMPHOCYTE
Morphology:	EPITHELIAL
Pathology:
Scientific Name:	MUS MUSCULUS
Vulgar Name:	MOUSE
Sex:	MALE
Miscelaneous Info:
Virus Succeptibility:	HERPES SIMPLEX VIRUS; PSEUDORABIES VIRUS; VACCINIA VIRUS; HUMAN POLIOVIRUS 1;
Virus Resistance:
Tumor Formation:
Products:	steroid hormones
Dependency Isoenzymes:
Culture Medium:	Complete growth medium: The base medium for this cell line is F-12K Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: At least 2 times per week (or else hormone production will decrease) Storage temperature: liquid nitrogen vapor temperature
Consigner:	Dr. Alain Zweibaum, INSERM, France; through Dr. Joao R. C. Andrade, FCM, UERJ, Rio de Janeiro.
Reference:	1216: Yasumura Y, et al. Clonal analysis of differentiated function in animal cell cultures. I. Possible correlated maintenance of differentiated function and the diploid karyotype. Cancer Res. 26: 529-535, 1966. PubMed: 5930699 21447: American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992. 21556: U.S. Food & Drug AdministrationDetection of cholera enterotoxin (CT) and cytotoxinIn: U.S. Food & Drug AdministrationBacteriological analytical manual8th ed.Gaithersburg, MDAOAC Internationalpp. 9.12-9.15, 1995 23251: Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682 26234: Cohen AI, et al. Histologic and physiologic characteristics of hormone-secreting transplantable adrenal tumors in mice and rats. Am. J. Pathol. 33: 631-651, 1957. PubMed: 13444454 26235: Cohen AI, et al. In vitro response of functional experimental adrenal tumors to corticotropin ACTH. Proc. Soc. Exp. Biol. Med. 95: 304-309, 1957. PubMed: 13441719
Additional Info:	Steroid secretion is stimulated by ACTH. Tested and found negative for ectromelia virus (mousepox). Cytogenetic Analysis: modal number = 41; range = 40 to 108. Stemline number is hyperdiploid. Karyotype stable within stemline number. Thirty-nine chromosomes with terminal centromeres plus 2 minute chromosomes; some cells with only l minute chromosome. The first chromosome is quite large and some of the smaller chromosomes are smaller than normal, but no specific structural abnormalities were detected Virus Susceptibility: Herpes simplex virus Pseudorabies virus Vaccinia virus Vesicular stomatitis virus Human poliovirus 1
ATCC:	CCL-79
Biosafety:	1