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BCRJ:	0253
Cell Line:	XR63-IL5
Tissue:	HEMATOPOIETIC
Organ:
Cell Type:	MYELOID
Morphology:	LYMPHOBLAST
Pathology:	MYELOMA
Scientific Name:	MUS MUSCULUS
Vulgar Name:	MOUSE
Sex:
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:
Products:	Interleukin-5; IL-5
Dependency Isoenzymes:
Culture Medium:	RPMI-1640 medium 90%, fetal bovine serum 10%. Subculture procedure: maintain cultures from 1x10E5 to 1x10E6 cells/mL.
Consigner:	RPMI-1640 medium 90%, fetal bovine serum 10%. Subculture procedure: maintain cultures from 1x10E5 to 1x10E6 cells/mL.
Reference:	Eur. J. Immunol. 18: 97-104, 1988.
Additional Info:	XR63-IL5 cell line was derived from X63Ag-654 mouse myeloma. It was transformed by modified pBV-1MTHA vector containing IL-5 cDNA cloned by Kinashi et Al ( Nature, 1986. 324: 70 ). Transformants secrete mouse IL-5 at 10 fold higher titres than those produced by Concanavalin A stimulated 2.19 T cells, as judged by the proliferation and maturation of B cell leukemia BCL1. Passible spontaneous revertants should be eliminated by periodic culture of this cell line in the presence of 1mg/mL Geneticin.
ATCC:
Biosafety:	01