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BCRJ:	0252
Cell Line:	XR63-IL4
Tissue:	HEMATOPOIETIC
Organ:
Cell Type:	MYELOID
Morphology:	LYMPHOBLAST
Pathology:	MYELOMA
Scientific Name:	MUS MUSCULUS
Vulgar Name:	MOUSE
Sex:
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:
Products:	Interleukin-4; IL-4
Dependency Isoenzymes:
Culture Medium:	RPMI-1640 medium 90%, fetal bovine serum 10%. Subculture procedure: maintain cultures from 1x10E5 to 1x10E6 cells/mL.
Consigner:	Dr. Fritz Melchers , Basel Institute for Immunology, Basel, Switzerland.
Reference:	Eur. J. Immunol. 18: 97-104, 1988.
Additional Info:	XR63-Il4 was derived from X63Ag-654 myeloma cell line. It was transformed by pBV-1MTHA vector containing IL-4 cDNA cloned by NOMA ( Nature, 1986, 319:640 ). Transformants produce mouse IL-4 in titres 150 hiher than those produced by concanavalin A stimulating2.19 T cells. This IL-4 produced the 3H - thimidine uptake of T cell line, a mast cell line amd a B leukemia cells, and enhanced the production of IgG1 by B-cells. Possible spontaneous revertants should be eliminated by periodic culture of this cell line in the presence of 1mg/mL Geneticin.
ATCC:
Biosafety:	01