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BCRJ:	0251
Cell Line:	XR63-IL3
Tissue:	HEMATOPOIETIC
Organ:
Cell Type:	MYELOID
Morphology:	LYMPHOBLAST
Pathology:	MYELOMA
Scientific Name:	MUS MUSCULUS
Vulgar Name:	MOUSE
Sex:
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:
Products:	Interleukin-3; IL-3
Dependency Isoenzymes:
Culture Medium:	RPMI-1640medium 90%, fetal bovine serum 10%. Subculture procedure: maintain cultures from 1x105 to 1x106 cells/mL.
Consigner:	Dr. Fritz Melchers, Basel Institute for Immunology, Basel Switzeland.
Reference:	Eur J Immunol 18:97-104, 1988.
Additional Info:	XR63-IL3 was derived from X63AG-654 myeloma cell line. It was transformed py pBV-1MTHA vector containing IL-3 cDNA cloned by Noma ( Nature, 1986, 319:640). Transformants produce mouse IL-3 in titres higher than those produced by concanavalin A stimulating T cells. This IL-3 produced the 3H-Thymidine uptake of T-cell line, a mast cell line and B leukemia cells, and enhanced the productions of IgG1 by B-cells. Possible spontaneous revertants should be eliminated by periodic culture of this cell line in the presence of 1mg/mL Geneticin. May be distributed to scientific institutions; not to distributed for any commercial use.
ATCC:
Biosafety:	01