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BCRJ:	0250
Cell Line:	XR63-IL2
Tissue:	HEMATOPOIETIC
Organ:
Cell Type:	MYELOID
Morphology:	LYMPHOBLAST
Pathology:	MYELOMA
Scientific Name:	MUS MUSCULUS
Vulgar Name:	MOUSE
Sex:
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:
Products:	Interleukin-2; IL-2
Dependency Isoenzymes:
Culture Medium:	RPMI-1640 medium 90%, fetal bovine serum 10%. Subculture procedure: maintain cultures from 1x10E5 to 1x10E6 cells/mL.
Consigner:	Dr. Fritz Melchers , Basel Institute for Immunology, Basel, Switzerland.
Reference:	Eur. J. Immunol. 18: 97-104, 1988.
Additional Info:	XR63-IL2 cell line was derived from X63Ag-654 myeloma cell line. It was transformed by pBV-1MTHA vecctor containing IL-2 ccDNA cloned by Dr. W. Fiers (State University of Ghent, Belgium). cells stably carry 30-100 copies of the plasmid per cell and constitutively secrete biolocally active mouse IL-2 in quantities similar to EL-4 thymoma cells and rat spleen cells stimulated with mitogens. Possible spontaneous revertants should be eliminated by periodic culture of this cell line in the presence of 1 mg/mL Geneticin.
ATCC:
Biosafety:	01