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| BCRJ: | 0248 | | Cell Line: | WEHI-3B | | Tissue: | HEMATOPOIETIC | | Organ: | PERIPHERAL BLOOD | | Cell Type: | MONOCYTE | | Morphology: | MACROPHAGE | | Pathology: | LEUKEMIA | | Scientific Name: | MUS MUSCULUS | | Vulgar Name: | MOUSE; BALB/C MOUSE | | Sex: | | | Miscelaneous Info: | | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | Lysozyme; granulocyte colony stimulating factor; G-CSF; interleukin-3; IL-3 | | Dependency Isoenzymes: | | | Culture Medium: | Dulbecco's MEM 90%, fetal bovine serum 10%.
Subculture procedure: dislodge the cells from the substrate flask by
scraping, and dispense into new flasks with fresh medium. a split
ratio of 1:4 to 1:8 is recommended | | Consigner: | J.M. Heard, Laboratoire d'Immunologie et virologie de Tumeurs,
hospital Cochin, Paris, through Dr. Christine Blaineau, Instituto
de Biofisica, UFRJ, Rio de Janeiro. | | Reference: | Cancer Res. 37: 546-550, 1977;
Differentiation 11; 59-63, 1978;
Blood 59: 761-767, 1982 | | Additional Info: | This myelomonocytic leukemia macrophage-like cell line was
derived from a BALB/c mouse. It produces the contitutive enzyme
lysozyme, interleukin-3 and the granulocyte colony-stimulating
activity (CSA). Its growth is inhibited by concentrations of LPS
as low as 4.0 ng/mL and blocked completely at higher
concentrations. Dextran sulfate also inhibits growth in
concentrations of 30 to 40 ug/mL. Production of lysozyme and CSA
is not inhibited or is actually enhanced during inhibition of
cell growth. The cell surface bears receptor for immunoglobulin
and complement. WEHI-3 lines exhibit only weak effector activity
against sheep erythrocytes or the tumor target EL-4 in an
antibody-dependent cell mediated cytotoxic system. | | ATCC: | TIB 68 | | Biosafety: | 01 |
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