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BCRJ:	0247
Cell Line:	WEHI 164
Tissue:	HEMATOPOIETIC
Organ:
Cell Type:	METHYLCHOLANTHRENE INDUCED
Morphology:	MACROPHAGE
Pathology:	FIBROSARCOMA
Scientific Name:	MUS MUSCULUS
Vulgar Name:	MOUSE; BALB/C MOUSE
Sex:
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:
Products:
Dependency Isoenzymes:
Culture Medium:	RPMI 1640 medium suplemented with 10%(v/v) fetal calf serum. Subculture procedures: cells must me scraped or dislodged form tissue culture flasks. A split ratio of 1:5 to 1:20 is recommended
Consigner:	Dr. Pedro Paulo Elsas Depto Imunologia Instituto de Microbiologia Paulo de Góes CCS/ UFRJ
Reference:	Blood, 65:8-14, 1985 Science, 230:943-945, 1985 Proc. Soc. Exp. Biol. Med. 144:813-818, 1996
Additional Info:	After treatment with actinomycin D, these cells are extremely death-sensible to human TNF-alfa, lymphotoxin and human citotoxic lymphocites. May be used to quantify this factor in biological fluids. May be distributed for research purposes. Not for commercial use.
ATCC:	CRL-1751
Biosafety:	01