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| BCRJ: | 0244 | | Cell Line: | V79-4 | | Tissue: | | | Organ: | LUNG | | Cell Type: | FIBROBLAST | | Morphology: | FIBROBLAST | | Pathology: | NORMAL | | Scientific Name: | CRICETULUS BARABENSIS GRISEUS | | Vulgar Name: | HAMSTER; CHINESE HAMSTER | | Sex: | | | Miscelaneous Info: | | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | | | Dependency Isoenzymes: | | | Culture Medium: | Dulbecco's MEM 90%, fetal bovine serum 10%.
Subculture procedure: satandard trypsinization techniques.
A split ratio 1:6 is recommended. | | Consigner: | Antonio Martins Monteiro - Banco de Células do Rio de Janeiro | | Reference: | 21877: . Molecular cell genetics. New York: Wiley; 1985.
22642: Chu EH, et al. Mammalian cell genetics. I. Selection and characterization of mutations auxotrophic for L-glutamine or resistant to 8-azaguanine in Chinese hamster cells in vitro. Genetics 62: 359-377, 1969. PubMed: 5392645
23252: Chu EH, Malling HV. Mammalian cell genetics. II. Chemical induction of specific locus mutations in Chinese hamster cells in vitro. Proc. Natl. Acad. Sci. USA 61: 1306-1312, 1968. PubMed: 5249812
26264: . . Nature 184: 1293-1295, 1959.
26265: Ford DK, Yerganian G. Observations on the chromosomes of Chinese hamster cells in tissue culture. J. Natl. Cancer Inst. 21: 393-425, 1958. PubMed: 13576097
26275: . Molecular cell genetics. New York: Wiley; 1985.
32373: Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260 | | Additional Info: | This cell line was established after spontaneous transformation
of cell isolated from the lung of a normal male . The G1 phase in these
cells is either absent or very short. Consequently they have been
used in studies of the G1 growth period. V-79 cell have a short
doubling time, rangingfrom 8 to 10 hours. | | ATCC: | CCL-93 | | Biosafety: | 01 |
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