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BCRJ:	0241
Cell Line:	U-87 MG
Tissue:
Organ:	BRAIN
Cell Type:	EPITHELIAL
Morphology:	EPITHELIAL
Pathology:	GLIOBLASTOMA; ASTROCIYOMA
Scientific Name:	HOMO SAPIENS
Vulgar Name:	HUMAN
Sex:	FEMALE
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:	YES
Products:	Interleukin-3 (interleukin 3, IL-3) and granulocyte/macrophage colony stimulating factor (GM-CSF)
Dependency Isoenzymes:
Culture Medium:	The base medium for this cell line is Eagle's Minimum Essential Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Medium Renewal: 2 to 3 times per week
Consigner:	ANTONIO MARTINS MONTEIRO BCRJ
Reference:	22159: Beckman G, et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238-241, 1971. PubMed: 4332744 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 23094: Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221 23128: Ponten J, Macintyre EH. Long term culture of normal and neoplastic human glia. Acta Pathol. Microbiol. Scand. 74: 465-486, 1968. PubMed: 4313504 32901: Li YM, et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306
Additional Info:	Antigen Expression: Blood Type A, Rh+ Cytogenetic Analysis: This is a hypodiploid human cell line with the modal chromosome number of 44 occurring in 48% of cells. The rate of higher ploidy was 5.9%., Twelve markers were common to all cells, including der(1)t(1;3) (p22;q21), der(16)t(1;16) (p22;p12), del(9) (p13) and nine others. The marker der(1) had two copies in most cells. There was only one copy of normal X. N1, N6 and N9 were not found. Isoenzymes: AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 1 PGM1, 2 PGM3, 1
ATCC:	HTB-14
Biosafety:	1