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BCRJ:	0240
Cell Line:	U266B1 [U266]
Tissue:
Organ:	PERIPHERAL BLOOD
Cell Type:	B LYMPHOCYTE
Morphology:	LYMPHOBLAST
Pathology:	MYELOMA
Scientific Name:	HOMO SAPIENS
Vulgar Name:	HUMAN
Sex:	MALE
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:
Products:	immunoglobulin; monoclonal antibody; interleukin 6 (interleukin-6, IL-6)
Dependency Isoenzymes:
Culture Medium:	The base medium for this cell line is RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%. Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Consigner:	PRISCILA SEGGES INCA
Reference:	1180: Nilsson K, et al. Established immunoglobulin producing myeloma (IgE) and lymphoblastoid (IgG) cell lines from an IgE myeloma patient. Clin. Exp. Immunol. 7: 477-489, 1970. PubMed: 4097745 22377: Kawano M, et al. Autocrine generation and requirement of BSF-2/IL-6 for human multiple myelomas. Nature 332: 83-85, 1988. PubMed: 3258060 32396: Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128
Additional Info:	U266 cells have been reported to produce human IL-6. DNA Profile (STR): Amelogenin: X,Y CSF1PO: 12,13 D13S317: 12 D16S539: 10 D5S818: 11,12 D7S820: 11,12 THO1: 5,7 TPOX: 8 vWA: 17
ATCC:	TIB-196
Biosafety:	01