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BCRJ:	0239
Cell Line:	U 138 MG
Tissue:
Organ:	BRAIN
Cell Type:	B LYMPHOCYTE
Morphology:	POLYGONAL
Pathology:	GLIOBLASTOMA
Scientific Name:	HOMO SAPIENS
Vulgar Name:	HUMAN
Sex:
Miscelaneous Info:	The two glioblastoma cell lines, U-118 MG (HTB-15) and U-138 MG (HTB-16), reportedly from different individuals have identical VNTR and similar STR patterns. U-118 MG and U-138 MG are very similar cytogenetically and share at least six derivative marker chromosomes.
Virus Succeptibility:
Virus Resistance:
Tumor Formation:
Products:
Dependency Isoenzymes:
Culture Medium:	Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10% Subculture procedure: standard trypsinization techniques. A split ratio 1:4 to 1:8 is recommended
Consigner:	Ana Maria Oliveira Battastini, Bioquímica Universidade Federal do Rio Grande do Sul.
Reference:	Beckman G , et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238-241, 1971. Fogh J , et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
Additional Info:	The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. Isoenzymes Me-2, 1; PGM1, 1; PGM3, 1; ES-D, 1; AK-1, 1; GLO-1, 1-2; G6PD, B; Phenotype Frequency Product: 0.0511
ATCC:	HTB-16
Biosafety:	01