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BCRJ:	0235
Cell Line:	TOLEDO
Tissue:
Organ:	PERIPHERAL BLOOD
Cell Type:	B LYMPHOCYTE
Morphology:	LYMPHOBLAST
Pathology:	LYMPHOMA
Scientific Name:	HOMO SAPIENS
Vulgar Name:	HUMAN
Sex:	FEMALE
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:
Products:
Dependency Isoenzymes:
Culture Medium:	The base medium for this cell line is RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 X 10(5) viable cells/ml. Maintain cultures at cell concentrations between 3 X 10(5) and 3 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Doubling Time: 24 to 30 hrs Storage temperature: liquid nitrogen vapor phase
Consigner:	RAQUEL MAIA INCA
Reference:	53289: Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273
Additional Info:	Toledo was established in 1990 from peripheral blood leukocytes (PBL) of a patient that originally had a diffuse large cell lymphoma (DLCL). [53289] Following high-dose chemotherapy and bone marrow transplantation, the patient subsequently developed a lymphoma in the brain. [53289] Though the morphology of the cells resembles Burkitt's lymphoma, the cells lack the typical chromosomal translocations of Burkitt's lymphoma. The karyotype does exhibit multiple chromosomal aberrations. [53289] The cells do not express surface or cytoplasmic immunoglobulin. [53289] The cells are negative for Epstein-Barr virus. [53289] This cell line is a model system for studying non-Hodgkin lymphomas. Antigen Expression: CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 - [53289] DNA Profile (STR): D5S818:10,12 D7S820: 9,10 THO1: 8,9.3 TPOX: 8,11 vWA: 16,17 Amelogenin: X CSF1PO: 12 D13S317:11 D16S539:9
ATCC:	CRL-2631
Biosafety:	1