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BCRJ: | 0227 | Cell Line: | SP2/0-AG14 | Tissue: | HEMATOPOIETIC | Organ: | | Cell Type: | MYELOID | Morphology: | MYELOID | Pathology: | MYELOMA | Scientific Name: | MUS MUSCULUS | Vulgar Name: | MOUSE; BALB/C | Sex: | | Miscelaneous Info: | | Virus Succeptibility: | | Virus Resistance: | | Tumor Formation: | | Products: | | Dependency Isoenzymes: | | Culture Medium: | Dulbecco's MEM with 4.5 g/L glucose 90%, fetal bovine serum 10%.
Subculture procedure: maintain cultures from 1x10E5 to 1x10E6 cells/mL. | Consigner: | Instituto Oswaldo Cruz, Rio de Janeiro. | Reference: | Nature 276: 269-, 1978;
J. Immunol. 126: 317-321, 1981. | Additional Info: | Sp2/0-Ag14 is a non-secreting mouse myeloma. It is used for
hybridization with plasmocytes to obtain antibody-producing cell
lines. This myeloma is resistant 8-azaguanine at 20 ug/mL and does
not grow in HAT medium. Sp2/0-Ag14 was isolated as re-clone of
Sp2/HL-Ag wich was derived from Sp2/HLGK, a hybrid between a
BALB/c spleen cell with anti-sheep red blood cell activity and the
myeloma cell line P3X63Ag8. | ATCC: | CRL 1581 | Biosafety: | 01 |
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