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BCRJ: 0227
Cell Line: SP2/0-AG14
Tissue: HEMATOPOIETIC
Organ:
Cell Type: MYELOID
Morphology: MYELOID
Pathology: MYELOMA
Scientific Name: MUS MUSCULUS
Vulgar Name: MOUSE; BALB/C
Sex:
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:
Products:
Dependency Isoenzymes:
Culture Medium: Dulbecco's MEM with 4.5 g/L glucose 90%, fetal bovine serum 10%. Subculture procedure: maintain cultures from 1x10E5 to 1x10E6 cells/mL.
Consigner: Instituto Oswaldo Cruz, Rio de Janeiro.
Reference: Nature 276: 269-, 1978; J. Immunol. 126: 317-321, 1981.
Additional Info: Sp2/0-Ag14 is a non-secreting mouse myeloma. It is used for hybridization with plasmocytes to obtain antibody-producing cell lines. This myeloma is resistant 8-azaguanine at 20 ug/mL and does not grow in HAT medium. Sp2/0-Ag14 was isolated as re-clone of Sp2/HL-Ag wich was derived from Sp2/HLGK, a hybrid between a BALB/c spleen cell with anti-sheep red blood cell activity and the myeloma cell line P3X63Ag8.
ATCC: CRL 1581
Biosafety: 01


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