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| BCRJ: | 0223 | | Cell Line: | SH-SY5Y | | Tissue: | | | Organ: | BRAIN | | Cell Type: | EPITHELIAL | | Morphology: | EPITHELIAL | | Pathology: | NEUROBLASTOMA | | Scientific Name: | HOMO SAPIENS | | Vulgar Name: | HUMAN | | Sex: | FEMALE | | Miscelaneous Info: | | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | | | Dependency Isoenzymes: | | | Culture Medium: | The base medium for this cell line is a 1:1 mixture of Eagle's Minimum Essential Medium, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: These cells grow as a mixture of floating and adherent cells. The cells grow as clusters of neuroblastic cells with multiple, short, fine cell processes (neurites). Cells will aggregate, form clumps and float.
Remove the medium with the floating cells, and recover the cells by centrifugation. Rinse the adherent cells with fresh 0.25% trypsin, 0.53 mM EDTA solution, add an additional 1 to 2 ml of trypsin solution, and let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate, combine with the floating cells recovered above and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:20 to 1:50 is recommended
Medium Renewal: Every 4 to 7 days
Storage temperature: liquid nitrogen vapor phase | | Consigner: | CAROLINA ALVARES DA CUNHA
CCS- UFRJ | | Reference: | 22554: Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. PubMed: 6137586
23032: Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. PubMed: 29704 | | Additional Info: | Derived from metastatic site: bone marrow
Antigen Expression: Blood Type A; Rh+
DNA Profile (STR):
Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18
Cytogenetic Analysis:
modal number = 47; the cells possess a unique marker comprised of a chromosome 1 with a complex insertion of an additional copy of a 1q segment into the long arm, resulting in trisomy of 1q [22554] | | ATCC: | CRL-2266 | | Biosafety: | 1 |
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