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BCRJ:	0217
Cell Line:	SAOS 2
Tissue:	BONE TISSUE
Organ:	BONE MARROW
Cell Type:	EPITHELIAL
Morphology:	EPITHELIAL
Pathology:	OSTEOSARCOMA
Scientific Name:	HOMO SAPIENS
Vulgar Name:	HUMAN
Sex:	FEMALE
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:
Products:
Dependency Isoenzymes:
Culture Medium:	McCoy's 5a medium (modified) with 1.5 mM L-glutamine adjusted to contain 2.2 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%. Subculture procedure: standard trypsinization techniques. A split ratio 1:4 to 1:6 is recommended.
Consigner:	Name: Dr. Marcos Farina de Souza Institution: Instituto de Ciências Biomédicas/ICB Centre: Centro de Ciências e Saúde/CCS Department: Departamento de Anatomia Laboratory: Laboratório de Biomineralização Address: Ilha do Fundão/UFRJ, Centro de Ciências da Saúde, BL F, 2° andar, sala F2027 Fone: + 55 (21) 25626393
Reference:	Banerjee C , et al. An AML-1 consensus sequence binds an osteoblast-specific complex and transcriptionally activates the osteoclacin gene. Proc. Natl. Acad. Sci. USA 93: 4968-4973, 1996. Fogh J, editor. Human tumor cells in vitro. 93: New York: Plenum Press; 1975, pp. 115-159. Fogh J , et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. Fogh J , et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. Takeuchi Y , et al. Relationship between actions of transforming growth factor (TGF)-beta and cell surface expression of its receptors in clonal osteoblastic cells. J. Cell. Physiol. 162: 315-321, 1995. Zhang W , et al. EGF-mediated phosphorylation of extracellular signal-regulated kinases in osteoblastic cells. J. Cell. Physiol. 162: 348-358, 1995. Pollack MS , et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. Schar BK , et al. Simultaneous detection of all four alkaline phosphatase isoenzymes in human germ cell tumors using reverse transcription-PCR. Cancer Res. 57: 3841-3846, 1997. Morris GF , et al. Transcriptional activation of the human proliferating-cell nuclear antigen promoter by p53. Proc. Natl. Acad. Sci. USA 93: 895-899, 1996. Werner H , et al. Wild-type and mutant p53 differentially regulate transcription of the insulin-like growth factor I receptor gene. Proc. Natl. Acad. Sci. USA 93: 8318-8323, 1996. Wang X , et al. Variabilin, a novel RGD-contining antagonist of glycoprotein IIb-IIIa and platelet aggregation inhibitor from the gard tick Dermacentor variabilis. J. Biol. Chem. 271: 17785-17790, 1996. Karnieli E , et al. The IGF-1 receptor gene promoter is a molecular target for the Ewing's Sarcoma=Wilms' Tumor 1 fusion protein. J. Biol. Chem. 271: 19304-19309, 1996.
Additional Info:	Isoenzymes: AK-1, 1; ES-D, 2; G6PD, B; GLO-I, 2; Me-2, 1; PGM1, 1-2; PGM3, 1-2 . This is one of an extensive series of human tumor lines isolated and characterized by J. Fogh and G. Trempe. The patient was treated with RTG, methotrexate, adriamycin, vincristine, cytoxan, and aramycin-C.
ATCC:	HTB-85
Biosafety:	01