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Results of Search
| BCRJ: | 0214 | | Cell Line: | RT-4 | | Tissue: | TRANSITIONAL CELL | | Organ: | BLADER | | Cell Type: | EPITHELIAL | | Morphology: | EPITHELIAL-LIKE | | Pathology: | | | Scientific Name: | HOMO SAPIENS | | Vulgar Name: | HUMAN | | Sex: | MALE | | Miscelaneous Info: | Disease: transitional cell papilloma | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | | | Dependency Isoenzymes: | | | Culture Medium: | McCoy's 5a medium (modified) with 1.5 mM L-glutamine adjusted to contain 2.2 g/L sodium bicarbonate, 90%; fetal bovine serum, 10%.
Subculture procedure: standard trypsinization techniques.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium renewal: 2 to 3 times per week | | Consigner: | Ana Maria Oliveira Battastini - Institution: UFRGS - Centre: Instituto de Ciências Básicas da Saúde - Department: Bioquímica
Laboratory: 22 - Address: Ramiro Barcelos, 2600-anexo Fone:(51) 3316 5553 Fax: (51) 3316 5535 | | Reference: | O'Toole C . Human bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitro. In: Webber, M.M.; Sekely, L.I., eds., editor. In vitro models for cancer research. vol. IV: Boca Raton, FL: CRC Press; pp. 103-125.
O'Toole C , et al. Cellular immunity to human urinary bladder carcinoma. I. Correlation to clinical stage and radiotherapy. Int. J. Cancer 10: 77-91, 1972. PubMed: 4196436
Fogh J , et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
Fogh J , et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
Lasfargues EY , et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572
Lasfargues EY , et al. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication. In Vitro 15: 723-729, 1979. PubMed: 94035
Rigby CC , Franks LM . A human tissue culture cell line from a transitional cell tumour of the urinary bladder: growth, chromosone pattern and ultrastructure. Br. J. Cancer 24: 746-754, 1970. PubMed: 5503601
Pollack MS , et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 | | Additional Info: | | | ATCC: | HTB-2 | | Biosafety: | 01 |
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