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BCRJ: 0211
Cell Line: RAJI
Tissue: HEMATOPOIETIC
Organ:
Cell Type: LYMPHOBLAST
Morphology: LYMPHOBLAST
Pathology: LYMPHOMA
Scientific Name: HOMO SAPIENS
Vulgar Name: HUMAN; NEGRO
Sex:
Miscelaneous Info:
Virus Succeptibility: VESICULAR STOMATITIS; POLIOMYELITIS ( BOTH PA
Virus Resistance:
Tumor Formation:
Products:
Dependency Isoenzymes:
Culture Medium: RPMI-1640 medium 90%, fetal bovine serum 10%. Subculture procedure: maintain cultures from 1x10E5 to 1x10E6 cells/mL
Consigner: Institute Pasteur, Paris, France.
Reference: 22142: Pulvertaft JV. Cytology of Burkitt's tumour (African lymphoma). Lancet 1: 238-240, 1964. PubMed: 14086209 22169: Epstein MA, Barr YM. Characteristics and mode of growth of tissue culture strain (EB1) of human lymphoblasts from Burkitt's lymphoma. J. Natl. Cancer Inst. 34: 231-240, 1965. PubMed: 14293790 22550: Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Cancer Inst. 65: 715-718, 1980. PubMed: 6932523 22572: Moore PS, et al. Primary characterization of a herpesvirus agent associated with Kaposi's sarcoma. J. Virol. 70: 549-558, 1996. PubMed: 8523568 26253: Epstein MA, et al. Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji). J. Natl. Cancer Inst. 37: 547-559, 1966. PubMed: 4288580 26254: . . Trans. N.Y. Acad. Sci. 29: 61, 1966. 32395: Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679 32448: Rich SA, et al. Purification, microresequencing, and immunolocalization of p36, a new interferon-alpha-induced protein that is associated with human lupus inclusions. J. Biol. Chem. 271: 1118-1126, 1996. PubMed: 8557639
Additional Info: The RAJI line of lymphoblast-like cells was established by R.Pulvertaft in 1963 from a Burkitt lymphoma of the left maxilla of an 11 year-old negro male. This cell line grows in suspension as single cells without attachment to glass, and as macroscopically visible clumps containing many hundrds of cells. Cells of RAJI line do not contain virus particles as demonstrated by electron microscopy.
ATCC: CCL-86
Biosafety: 02


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