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Results of Search
| BCRJ: | 0191 | | Cell Line: | NIH/3T3 | | Tissue: | EMBRYO | | Organ: | EMBRYO | | Cell Type: | FIBROBLAST | | Morphology: | FIBROBLAST | | Pathology: | NORMAL | | Scientific Name: | RATTUS NORVEGICUS | | Vulgar Name: | RAT; NIH SWISS ALBINO | | Sex: | | | Miscelaneous Info: | | | Virus Succeptibility: | MURINE SARCOMA VIRUS; MURINE LEUKEMIA VIRUS | | Virus Resistance: | | | Tumor Formation: | | | Products: | | | Dependency Isoenzymes: | | | Culture Medium: | Dulbecco's MEM 90%, fetal bovine serum 10%.
Subculture procedure: satandard trypsinization techiniques.
A split ratio of 1:3 to 1:6 is recommended. | | Consigner: | ATCC, through Dr. Wanderley de Souza, Instituto de Biofisica,
UFRJ, Rio de Janeiro. | | Reference: | J. Virol. 4: 549-553, 1969;
Cell 16: 63-75, 1979. | | Additional Info: | The NIH/3T3, acontinous cell line of highly contact-inhibited cells was
established from NIH Swiss mouse embryo cultures. This cell line is
usefull for DNA transfection and transformation studies. These cells
are highly sensitive to sarcoma virus focus formation and leukemia
virus propagation. It has a tendency to acquire spontaneously the
transformed phenotype along protuded in vitro culture. | | ATCC: | CRL 1658 | | Biosafety: | 01 |
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