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Results of Search
| BCRJ: | 0189 | | Cell Line: | NEURO-2A | | Tissue: | NEURAL | | Organ: | BRAIN | | Cell Type: | B LYMPHOCYTE | | Morphology: | NEURON-LIKE | | Pathology: | NEUROBLASTOMA | | Scientific Name: | MUS MUSCULUS | | Vulgar Name: | MOUSE; A ALBINO | | Sex: | | | Miscelaneous Info: | | | Virus Succeptibility: | VESICULAR STOMATITIS ( INDIANA STRAIN ) AND HERPES | | Virus Resistance: | POLIOVIRUS TYPE 1. | | Tumor Formation: | IN STRAIN A ALBINO MICE | | Products: | | | Dependency Isoenzymes: | | | Culture Medium: | Dulbecco's MEM with 4.5 g/L glucose 90%, fetal bovine serum 10%. Subculture
procedure: standard trypsinization techniques. A split ratio 1:3 is recommended. | | Consigner: | Dr. Elizabeth Obino Lima, Fundação Instituto Oswaldo Cruz, Rio de Janeiro. | | Reference: | J. Neurochem. 64(1): 451-4, 1995
J. Biol. Chem. 269(48): 30451-6, 1994 | | Additional Info: | Clone Neuro-2a was established by R.J.Klebe and F.H. Ruddle from a
spontaneous tumor of a strain A albino mouse. This tumor line, designated
C1300, was obtained from the Jackson Laboratory, Bar Harbor, Maine. The cells
were cloned in F2 medium ( Ham ), agamma bovine serum, 10%, penicillin ans
streptomycin and 0,125% agarose. The cloned cells were maintained in F12 or
MEM and 10% agamma bovine serum. Neuronal cells produce large qunatities of
microtubular protein which is belived to play a role in a nerve cell. May be
distributed to scientific institutions; not to distributed for any commercial use. | | ATCC: | CCL131 | | Biosafety: | 01 |
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