|
Results of Search
| BCRJ: | 0187 | | Cell Line: | NCI-H292 [H292] | | Tissue: | | | Organ: | LUNG | | Cell Type: | EPITHELIAL | | Morphology: | EPITHELIAL | | Pathology: | MUCOEPIDERMOID PULMONARY CARCINOMA | | Scientific Name: | HOMO SAPIENS | | Vulgar Name: | HUMAN | | Sex: | FEMALE | | Miscelaneous Info: | | | Virus Succeptibility: | Hepatitis B virus | | Virus Resistance: | | | Tumor Formation: | YES | | Products: | keratin; vimentin | | Dependency Isoenzymes: | | | Culture Medium: | The base medium for this cell line is RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 48 hrs | | Consigner: | JERSON LIMA DA SILVA, UFRJ Instituto de Bioquímica Médica - CCS | | Reference: | 1605: Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876
22946: Yoakum GH, et al. High-frequency transfection and cytopathology of the hepatitis B virus core antigen gene in human cells. Science 222: 385-389, 1983. PubMed: 6194563
23056: Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257 | | Additional Info: | This line was derived from a lymph node metastasis of a pulmonary mucoepidermoid carcinoma.
The cells were isolated in a chemically defined medium (HITES) and later adapted to growth in media supplemented with serum.
The cells retain their mucoepidermoid characteristics in culture as determined by their ultrastructure and expression of multiple markers of squamous differentiation.
The cells support the growth of hepatitis B virus and are negative for L-DOPA decarboxylase.
The line has been selected as a prototype for transfecting human subgenomic fragments into human cells for studying the role of HBV and its individual genes in the pathogenesis of viral hepatitis and liver cancer.
The cells stain positive for keratin and vimentin and are mucicarmine positive but are negative for neurofilament triplet protein.
DNA Profile (STR):
Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 9,13
D5S818: 13
D7S820: 10
THO1: 8
TPOX: 8,11
vWA: 16,17
Cytogenetic Analysis:
This is a human cell line with near-diploid chromosome counts. The modal chromosome number was 47, occurring in 36% of cells. The rate of cells with a higher ploidy count was 3.9%. Twelve markers were common to most cells. Among them were del(1) (q32.1), der (5)t(5;13) (p15.33;q11), i(5p), der(1)t(1;?) (p34.3;?) and der (6)t(6;7) (p25.3;q21.2). All markers were present in single copy per cell. Normal N1 and N6 were absent. There were two normal X chromosomes. No other abnormalities were detected. | | ATCC: | CRL-1848 | | Biosafety: | 1 |
|
