Results of Search
BCRJ: | 0185 | Cell Line: | NCI-H1299 | Tissue: | | Organ: | LUNG | Cell Type: | B LYMPHOCYTE | Morphology: | EPITHELIAL | Pathology: | CARCINOMA | Scientific Name: | HOMO SAPIENS | Vulgar Name: | HUMAN | Sex: | MALE | Miscelaneous Info: | | Virus Succeptibility: | | Virus Resistance: | | Tumor Formation: | | Products: | neuromedin B | Dependency Isoenzymes: | | Culture Medium: | The base medium for this cell line is RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subcultivation Ratio: 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Storage temperature: liquid nitrogen vapor phase | Consigner: | | Reference: | 23517: Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005
23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..
33177: Lin DL, Chang C. p53 is a mediator for radiation-repressed human TR2 orphan receptor expression in MCF-7 cells, a new pathway from tumor suppressor to member of the steroid receptor superfamily. J. Biol. Chem. 271: 14649-14652, 1996. PubMed: 8663350 | Additional Info: | The cells have a homozygous partial deletion of the p53 protein, and lack expression of p53 protein. They reported to be able to synthesize the peptide neuromedin B (NMB) at 0.1 pmol/mg protein, but not the gastrin releasing peptide (GRP).
DNA Profile (STR):
Amelogenin:X
CSF1PO:12
D13S317:12
D16S539:12,13
D5S818:11
D7S820:10
THO1:6,9.3
TPOX:8
vWA: 16,17,18 | ATCC: | CRL-5803 | Biosafety: | 1 |
|