| BCRJ: | 0183 |
| Cell Line: | NCF |
| Tissue: | CONNECTIVE |
| Organ: | HEART |
| Cell Type: | FIBROBLAST |
| Morphology: | FIBROBLAST |
| Pathology: | NORMAL |
| Scientific Name: | RATTUS NORVEGICUS; NEONATAL |
| Vulgar Name: | RAT; NEONATAL |
| Sex: | |
| Miscelaneous Info: | NEONANAL RAT |
| Virus Succeptibility: | |
| Virus Resistance: | |
| Tumor Formation: | |
| Products: | |
| Dependency Isoenzymes: | |
| Culture Medium: | RPMI 1640 medium suplemented with 10% (v/v) Fetal Calf Serum. Standard
tripsinization techniques. Split ratio from 1:2 to 1:3 recommended. |
| Consigner: | Dra. Regina Coeli dos Santos Goldenberg
Lab de Membranas Excitáveis
Programa de Fisiologia Celular e de Sistemas
Instituto de Biofísica Carlos Chagas Fo
CCS/UFRJ |
| Reference: | |
| Additional Info: | This primary cell culture were obtained by tripsin diggestion of entire rat heart
followed by collagenase type II treatment. Cells were partially purified by diferential
adesion to eliminate myocites for 2 hours and the supernadant plated after that.
Complete isolation protocol avaiable under request. |
| ATCC: | |
| Biosafety: | 01 |
