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BCRJ:	0174
Cell Line:	MIA PaCa 2
Tissue:	TUMOR TISSUE
Organ:	PANCREAS
Cell Type:	EPITHELIAL
Morphology:	EPITHELIAL
Pathology:	CARCINOMA
Scientific Name:	HOMO SAPIENS
Vulgar Name:	HUMAN
Sex:	MALE
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:
Products:	human colony stimulating factor, subclass I (CSF-I); plasminogen activator
Dependency Isoenzymes:
Culture Medium:	Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 87.5%; fetal bovine serum, 10%; horse serum, 2.5% Subculture procedure: standard trypsinization techniques. A split ratio 1:4 to 1:8 is recommended.
Consigner:	Ana Marisa Chudzinski-Tavassi - Instituto Butantan - Av. Vital Brasil 1500 - Fone: 55 11 37267222 - 2043
Reference:	1057: Wu M , et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590 22291: Fountzilas G , et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line (MIA PaCa-2). J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740 49623: Robertson JF , et al. Effect of gastrointestinal hormones and synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455 58034: Yunis AA , et al. Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase. Int. J. Cancer 19: 128-135, 1977. PubMed: 832918
Additional Info:	Organ: pancreas - The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male. The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximately 19%. Hybridomas have been made against the CSF-I produced by this line ATCC HB-8207 (F1A3-23) and ATCC HB-8208 (F18 AF1). The line is sensitive to asparaginase.
ATCC:	CRL-1420
Biosafety:	01