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| BCRJ: | 0173 | | Cell Line: | MG-63 | | Tissue: | BONE TISSUE | | Organ: | BONE | | Cell Type: | B LYMPHOCYTE | | Morphology: | FIBROBLAST | | Pathology: | OSTEOSARCOMA | | Scientific Name: | HOMO SAPIENS | | Vulgar Name: | HUMAN | | Sex: | MALE | | Miscelaneous Info: | | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | interferon | | Dependency Isoenzymes: | | | Culture Medium: | The base medium for this cell line is Eagle's Minimum Essential Medium. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Storage temperature: liquid nitrogen vapor phase | | Consigner: | MAIA SILVA VICCARI GATTI
UNIVERSIDADE ESTADUAL DE CAMPINAS | | Reference: | -- | | Additional Info: | Receptors:
transforming growth factor beta (TGF-beta) RI, expressed
transforming growth factor beta (TGF-beta) RII, expressed
DNA Profile (STR):
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 9.3
TPOX: 8,11
vWA: 16,19
Cytogenetic Analysis:
This is a hypotriploid human cell line. The modal chromosome number was 66 occurring in 44% of cells. The rate of cells with higher ploidies was 2.0%. Eighteen to 19 marker chromosomes were common to all cells. | | ATCC: | CRL-1427 | | Biosafety: | 1 |
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