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BCRJ:	0168
Cell Line:	MDCK (NBL-2)
Tissue:	EPITHELIUM
Organ:	KIDNEY
Cell Type:	EPITHELIAL
Morphology:	EPITHELIAL
Pathology:	NORMAL
Scientific Name:	CANIS FAMILIARIS
Vulgar Name:	DOG; COCKER SPANIEL
Sex:	FEMALE
Miscelaneous Info:	ADULT
Virus Succeptibility:	VESICULAR STOMATITIS (INDIANA); VACCINIA; COXSACKI
Virus Resistance:	POLIOVIRUS; COXSACKIE VIRUS B3 AND B4
Tumor Formation:
Products:	Keratin
Dependency Isoenzymes:
Culture Medium:	Dulbecco's MEM 90%, fetal bovine serum 10%. Subculture procedure: standard trypsinization techiniques. A split ratio 1:4 is recommended.
Consigner:	Dr. Hiroshi Mizusawa, Japan Cancer Resources Bank, Tokyo, Japan; through Dr. Alvaro Monteiro, Departamento de Bioquimica, Instituto de Quimica, UFRJ.
Reference:	Proc. Soc. Exp. Med. 98: 574-, 1958.
Additional Info:	MDCK was derived from a kidney of normal adult, female Cocker Spaniel in 1958 by S. H. Madin and N. B. Darby (Mardin Darby Canine Kidney). The cell line found to support the growth of a wide range of animal viruses.
ATCC:	CCL 34
Biosafety:	01