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BCRJ:	0166
Cell Line:	MDA-MB-468
Tissue:
Organ:	MAMARIAN GLAND
Cell Type:	EPITHELIAL
Morphology:	EPITHELIAL
Pathology:	ADENOCARCINOMA
Scientific Name:	HOMO SAPIENS
Vulgar Name:	HUMAN
Sex:	FEMALE
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:	YES
Products:
Dependency Isoenzymes:
Culture Medium:	The base medium for this cell line is Leibovitz's L-15 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 100% Temperature: 37.0°C Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week Storage temperature: liquid nitrogen vapor phase
Consigner:	MARILIA MEIRA DIAS UNIVERSIDADE ESTADUAL DE CAMPINAS
Reference:	1206: Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337 22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779 22544: Pathak S, et al. A human breast adenocarcinoma with chromosome and isoenzyme markers similar to those of the HeLa line. J. Natl. Cancer Inst. 62: 263-271, 1979. PubMed: 283262 22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202 22930: Nigro JM, et al. Mutations in the p53 gene occur in diverse human tumour types. Nature 342: 705-707, 1989. PubMed: 2531845 22977: Bates SE, et al. Expression of the transforming growth factor-alpha/epidermal growth factor receptor pathway in normal human breast epithelial cells. Endocrinology 126: 596-607, 1990. PubMed: 2294006 23111: Avila MA, et al. Quercetin mediates the down-regulation of mutant p53 in the human breast cancer cell line MDA-MB468. Cancer Res. 54: 2424-2428, 1994. PubMed: 8162591 32275: Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764 32467: Zamora-Leon SP, et al. Expression of the fructose transporter GLUT5 in human breast cancer. Proc. Natl. Acad. Sci. USA 93: 1847-1852, 1996. PubMed: 8700847
Additional Info:	The MDA-MB-468 cell line was isolated in 1977 by R. Cailleau, et al., from a pleural effusion of a 51-year-old Black female patient with metastatic adenocarcinoma of the breast. Although the tissue donor was heterozygous for the G6PD alleles, the cell line consistently showed only the G6PD A phenotype.There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.EGF receptor is present at 1 X 10(6) per cell. DNA Profile (STR): Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9 D5S818: 12 D7S820: 8 THO1: 7 TPOX: 8,9 vWA: 18 Cytogenetic Analysis: modal number = 64; range = 60 to 67. The cell line is aneuploid human, presumably female (X, abnormal X) with most chromosome counts in the hypotriploid range.; Normal chromosomes X, N2, N3, N7, N8, N10, and N22 are clearly under-represented due to their involvement in the formation of the many marker (19) chromosomes present in this cell line.; A normal chromosome N1 (or two) is identified in each karyotype, but, in addition, regions of chromosome N1 are also present in five different marker chromosomes.; Variation is evident in the normal and marker chromosome copy number from karyotype to karyotype. Isoenzymes: AK-1, 1 ES-D, 1 G6PD, A GLO-I, 1-2 Me-2, 1-2 PGM1, 1 PGM3, 2
ATCC:	HTB-132
Biosafety:	1