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BCRJ: | 0162 | Cell Line: | MCF-7 | Tissue: | EPITHELIUM | Organ: | BREAST | Cell Type: | EPITHELIAL | Morphology: | EPITHELIAL | Pathology: | ADENOCARCINOMA | Scientific Name: | HOMO SAPIENS | Vulgar Name: | HUMAN | Sex: | FEMALE | Miscelaneous Info: | CAUCASIAN, 69 YEAR OLD | Virus Succeptibility: | | Virus Resistance: | | Tumor Formation: | | Products: | Keratin | Dependency Isoenzymes: | | Culture Medium: | RPMI 1640 medium suplemented with 20% of fetal calf serum. Standard
tripsinization techniques. Split ratio rom 1:5 to 1:8 is recommended. | Consigner: | Dr. Ricardo Bretani
Ludwick Institute for Cancer Research
São Paulo - SP | Reference: | J.Nat. Canc. Inst., 51:1409, 1973
Cancer Res., 53:5882, 1993 | Additional Info: | This cell line was isolated from pleural effusion from 69 year female Caucasian.
Cells exhibit some features of differentiated mammary epithelium including
oestradiol synthesis and formation of domes. Cells may carry B or C type
retrovirus and is consiered to represent a category 2 pathogen. Cells express both
wildtype and variant oestrogen receptorss as well as progesterone receptors.
Restricted distribution. Only to be distributed with formal authorization of Dr.
Bretani. | ATCC: | HTB-22 | Biosafety: | 01 |
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