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BCRJ: | 0161 | Cell Line: | MCF 10A | Tissue: | | Organ: | MAMARIAN GLAND | Cell Type: | EPITHELIAL | Morphology: | EPITHELIAL | Pathology: | FIBROCYSTIC DISEASE | Scientific Name: | HOMO SAPIENS | Vulgar Name: | HUMAN | Sex: | FEMALE | Miscelaneous Info: | | Virus Succeptibility: | | Virus Resistance: | | Tumor Formation: | NO | Products: | | Dependency Isoenzymes: | | Culture Medium: | The base medium for this cell line is MEBM, which is supplied as part of the MEGM Bullet Kit. To make the complete growth medium, add the following components to the base medium: All MEGM SingleQuot additives that are supplied with the kit except the GA-1000 (BPE 13 mg/ml, 2 ml; hydrocortisone 0.5 mg/ml, 0.5 ml; hEGF 10 ug/ml, 0.5 ml; insulin 5 mg/ml, 0.5 ml); 100 ng/ml cholera toxin (sold separately).
Subculturing: Remove medium and rinse monolayer with PBS (ATCC Cat# 30-2200).Add 3.0 ml 0.05% trypsin, 0.53 mM EDTA and incubate at 37C for 15 minutes. To neutralize trypsin, add 3 ml solution of 0.1% soybean trypsin inhibitor.Centrifuge cell suspension at 125 xg for 5 to 10 minutes.Resuspend cell pellet in complete culture medium. Add appropriate aliquots of cell suspension to new culture vessels.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Storage temperature: liquid nitrogen vapor phase | Consigner: | MARIA LUCIA ZAIDAN
USP | Reference: | 21968: Soule H, McGrath CM. Immortal human mammary epithelial cell lines. US Patent 5,026,637 dated Jun 25 1991
22025: Pauley RJ, et al. Immortal human mammary epithelial cell sublines. US Patent 5,206,165 dated Apr 27 1993
22248: Soule HD, McGrath CM. A simplified method for passage and long-term growth of human mammary epithelial cells. In Vitro Cell. Dev. Biol. 22: 6-12, 1986. PubMed: 2418007
23084: Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed: 1975513
23085: Tait L, et al. Ultrastructural and immunocytochemical characterization of an immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6087-6094, 1990. PubMed: 1697506 | Additional Info: | The MCF 10A cell line is a non-tumorigenic epithelial cell line. [21968]
The line was produced by long term culture in serum free medium with low Ca++ concentration. [21968]
MCF 10A was derived from adherent cells in the population. [21968]
Cells derived from a floating population are available (see MCF 10F, ATCC CRL-10318). [21968]
The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen. [21968]
They exhibit three dimensional growth in collagen, and form domes in confluent cultures. [21968]
Thus far, the cells have shown no signs of terminal differentiation or senescence.
The line is responsive to insulin, glucocorticoids, cholera endotoxin, and epidermal growth factor (EGF). [21968]
By electron microscopy the cells display characteristics of luminal ductal cells but not of myoepithelial cells. [23085]
They also express breast specific antigens as detected by positive reaction with MFA-Breast and MC-5 monoclonal antibodies. [23085]
The calcium content of the medium exerts a strong effect on the morphology of the cells. [22248]
DNA Profile (STR):
Amelogenin: X
CSF1PO: 10,12
D13S317: 8,9
D16S539: 11,12
D5S818: 10,13
D7S820: 10,11
THO1: 8,9.3
TPOX: 9,11
vWA: 15,17
Isoenzymes:
AK-1, 1 [23084]
ES-D, 1 [23084]
G6PD, B [23084]
GLO-I, 1-2 [23084]
PGM1, 1-2 [23084]
PGM3, 1 [23084] | ATCC: | CRL-10317 | Biosafety: | 1 |
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