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BCRJ:	0159
Cell Line:	MCA-RH7777
Tissue:
Organ:	LIVER
Cell Type:	EPITHELIAL-LIKE
Morphology:	EPITHELIAL
Pathology:	HEPATOMA
Scientific Name:	RATTUS NORVEGICUS
Vulgar Name:	RAT
Sex:	FEMALE
Miscelaneous Info:	Addition of glucocorticoids (dexamethasone) to the medium accelerates cell proliferation and reduces alpha fetoprotein production. The cells tend to shed from the growth surface before becoming confluent.
Virus Succeptibility:
Virus Resistance:
Tumor Formation:	HEPATOMA, MORRIS HEPATOMA
Products:	alpha-fetoprotein (AFP, alpha fetoprotein)
Dependency Isoenzymes:
Culture Medium:	Dulbecco's modified Eagle's medium with 4 mM L-glutamine, 4.5 g/L glucose, 75%; horse serum, 20%; fetal bovine serum, 5%. Subculture procedure: standard trypsinization techniques.A split ratio 1:2 to 1:6 is recommended.
Consigner:	Michele Mamprim Grippa, Laboratório Experimental de Cultivo Celular - Centro de Ciências Biológicas – Pontifica Universidade Católica do Paraná.
Reference:	Recent Results Cancer Res. 44: 103-114, 1974. Kulas DT , et al. The transmembrane protein-tyrosine phosphatase LAR modulates signaling by multiple receptor tyrosine kinases. J. Biol. Chem. 271: 748-754, 1996. Schock D , et al. An auxiliary factor containing a 240-kDa protein complex is involved in apolipoprotein B RNA editing. Proc. Natl. Acad. Sci. USA 93: 1097-1102, 1996. Becker JE , et al.. Two new rat hepatoma cell lines for studying the unbalanced blocked ontogeny hypothesis. In: Fishman, W.H.; Sell, S., eds. , editor. . Onco-developmental gene expression. New York: Academic Press; 1976, pp. 259-270.
Additional Info:	Growth Properties: adherent
ATCC:	CRL 1601
Biosafety:	01