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BCRJ:	0156
Cell Line:	M3/84.6.34
Tissue:	HEMATOPOIETIC
Organ:	SPLEEN
Cell Type:	LYMPHOBLAST
Morphology:	LYMPHOBLAST
Pathology:	HYBRIDOMA
Scientific Name:	RATTUS NORVEGICUS
Vulgar Name:	RAT; LEWIS-X-BN F1
Sex:
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:
Products:	Immunoglobulin; monoclonal antibody; macrophage: ab against; Mac-3: ab against; IgG1-k
Dependency Isoenzymes:
Culture Medium:	RPMI-1640 80%, fetal bovine serum 20%. Subculture procedure: maintain cultures from 1x10E5 to 1x10E6 cells/mL.
Consigner:	American Type Culture Collection (Batch 13-X), Rockville, Maryland, USA.
Reference:	J. Biol. Chem. 256: 3833-39, 1981.
Additional Info:	Hybridoma developed by T. Springer et cols. Secretes rat IgG1 k antibodies that identify a 110,000 MW glycoprotein found on mouse mononuclear phagocytes, designated Mac-3 (macrophage subpopulation-specific antigen). Mac-2 and Mac-3 are not expressed on bone marrow cells. Mac-3 antibodies label 69% of spleen cells and 0-2% of thymocytes. Expression of antigen is increased during differentiation of monocytes to activated peritoneal macrophages. This hybridoma was formed by fusion of the mouse myeloma line P3-NSI/1-Ag4-1 with the spleen cells from (Lewis X BN) F1 rats immunized with detergent-solubilized murine (CC57Bl/6) macrophage membrane wich had been immunodepleted. The immunodepletion was accomplished with monoclonal antibody immunosorbents and removed previosly identified antigen prior to immunization of rats, thus increasing the probability of identifying a new antigen. Appropriate acknowledgement of the origin of this cell line should be made in all relevant publication. May be distributed toi scientific non-profit institutions . Not to be distributed for any commercial use.
ATCC:	TIB 168
Biosafety:	01