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BCRJ:	0155
Cell Line:	M3/38.1.2.8.HL.2
Tissue:	HEMATOPOIETIC
Organ:	SPLEEN
Cell Type:	LYMPHOBLAST
Morphology:	LYMPHOBLAST
Pathology:	HYBRIDOMA
Scientific Name:	RATTUS NORVEGICUS
Vulgar Name:	RAT; LEWIS-X-BN F1
Sex:
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:
Products:	Immunoglobulin; monoclonal antibody; macrophage: ab against; Mac-2: ab against; IgG2a-k
Dependency Isoenzymes:
Culture Medium:	RPMI-1640 80%, fetal bovine serum 20%. Subculture procedure: maintain cultures from 1x10E5 to 1x10E6 cells/mL.
Consigner:	American Type Culture Collection (Batch F-6165), Rockville, Maryland, USA.
Reference:	J. Immunol. 128: 1221-28, 1982; J. Biol. Chem. 256: 3833-39, 1981.
Additional Info:	This hybridoma secretes a monoclonal rat IgG2a K which identifies a 32,000 MW glicoprotein found on mononuclear phagocytes. This antigen has been designated as Mac-2. Mac-2 ( and Mac-3 ), in contrast to Mac-1, are not expressed on bone marrow cells. Thus, Mac-2 and Mac-3 appear to be expressed on the monocytic line of differentiation at some stage after divergence from granulocytic line, while Mac-1 is found on both branches. Mac-2 and Mac-3 antibodies label 69% of spleen cells and0-2% of thymocytes. Expression of sntigens is increased during differentiation of monocytes to activated peritoneal macrophages.
ATCC:	TIB 166
Biosafety:	01