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BCRJ:	0154
Cell Line:	M3
Tissue:	GLANDULAR
Organ:	MAMARIAN GLAND
Cell Type:	EPITHELIAL
Morphology:	EPITHELIAL
Pathology:	ADENOCARCINOMA
Scientific Name:	MUS MUSCULUS
Vulgar Name:	MOUSE, BALB/C
Sex:	FEMALE
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:
Products:	GM-CSF; murine GM-CSG.
Dependency Isoenzymes:
Culture Medium:	Dulbecco's MEM 90%, fetal bovine serum 10%. Subculture procedure: standard tripsinization techniques. A split ratio 1:2 is recommended.
Consigner:	Dr. Elisa Bal de Kier Joffú, Instituto de Oncologia "Angel H. Roffo " Universidad de Buenos Aires, Avenida San Martin 1417, Buenos Aires.
Reference:
Additional Info:	This Cell line is derived from an adenocarcinoma of mammary glandthat arouse spontaneously in a BALB/c mouse. this carcinoma can be maintained in vivo by subcutaneously implant of 5x10E6 tumor cells: 100% mice will develop tumo and die after 45-50 days. More than 60% animals have metastases to lungs. This tumor has a low immunogenicity. The cell line M3 was adapted to in vitro culture. It secretes constitutively GM-CSF, and the supernatant of these cells sustain proliferation of GM-CSF dependent cell lines.
ATCC:
Biosafety:	01