| BCRJ: | 0152 |
| Cell Line: | M1/70.15.11.5.HL |
| Tissue: | HEMATOPOIETIC |
| Organ: | SPLEEN |
| Cell Type: | LYMPHOBLAST |
| Morphology: | LYMPHOBLAST |
| Pathology: | HYBRIDOMA |
| Scientific Name: | RATTUS NORVEGICUS |
| Vulgar Name: | RAT; DA |
| Sex: | |
| Miscelaneous Info: | |
| Virus Succeptibility: | |
| Virus Resistance: | |
| Tumor Formation: | |
| Products: | Immunoglobulin: monoclonal antibody; macrophage: ab against; Mac-1:ag
against; IgG2b |
| Dependency Isoenzymes: | |
| Culture Medium: | RPMI-1640 medium 90%, fetal bovine serum 10%.
Subculture procedure: maintain cultures from 1x10E5 to 1x10E6 cells/mL. |
| Consigner: | American Type Culture Collection (batch F-5536), Rockville, Maryland,
USA. |
| Reference: | Eur. J. Immunol. 8: 539-551
Eur. J. Immunol. 9: 301-306
J. Exp. Med. 165: 733-749. |
| Additional Info: | Animal were immunized with C57BL/10 mouse spleen cells enriched
for lymphocytes. Spleen cell cells were fused with NS-1 myeloma cells.
Mac-1 is a mouse macrophage differentiation marker associated with
type-3 complement receptor (CR3). The antigen binding is expressed
in large amounts on thyoglicollate-induced peritoneal exudate
macrophages and in lesser quantities on neutrophilicc granulocytes
and thymus cell. This antibody precipitates two polypeptides of 190.000
and 105.000 daltons. It binds to human monocytes, polymorphonuclear
leucocytes ans small population of lymphocytes. The antibody is
capable of both natural killing and antibody dependent cytotoxicity.
May be distributed to scientific non-profit institutions, not to be
distributed for any commercial use. |
| ATCC: | TIB 128 |
| Biosafety: | 01 |
