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Results of Search
| BCRJ: | 0148 | | Cell Line: | L-M(TK-) | | Tissue: | CONNECTIVE | | Organ: | ADIPOSE | | Cell Type: | FIBROBLAST | | Morphology: | FIBROBLAST | | Pathology: | | | Scientific Name: | MUS MUSCULUS | | Vulgar Name: | MOUSE; C3H/AN | | Sex: | MALE | | Miscelaneous Info: | 100 DAYS OLD | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | IN NUDE MICE | | Products: | | | Dependency Isoenzymes: | | | Culture Medium: | | | Consigner: | National Institute of health, Bethesda, MD, USA; through Dr.Hector
Seuanez, Instituto Nacional do Cancer, Rio de Janeiro. | | Reference: | Exp. Cell Res. 31: 297-312, 1963;
Proc. Natl. Acad. Sci. 69: 510-514. | | Additional Info: | The L-M cell line was derived from L-929 cells. The L-M(TK-) was
derived from a 5-bromo-2-deoxyuridine (BrdU) resistant strain of
L-M cell line. Biochemical and radioautographic studies indicate
that this cell line no longer incorporated exogenous Brdu into
cellular DNA and the enzyme thymidine kinase was lacking. No
inhibition of growth potential was detected during culture in medium
supplemented with 60 ug/mL BrdU, and these cellos exhibit their
sensivity to HAT medium. they are therefore useful in somatic cell
hybridization studies. | | ATCC: | CCL 1.3 | | Biosafety: | 01 |
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