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| BCRJ: | 0141 | | Cell Line: | L6 | | Tissue: | SKELETAL MUSCLE | | Organ: | | | Cell Type: | MYOBLAST | | Morphology: | MYOBLASTIC-LIKE | | Pathology: | | | Scientific Name: | RATTUS NORVEGICUS | | Vulgar Name: | RAT | | Sex: | | | Miscelaneous Info: | | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | myosin | | Dependency Isoenzymes: | | | Culture Medium: | The base medium for this cell line is Dulbecco's Modified Eagle's Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Growth Conditions: The myoblastic component of this line will be depleted rapidly if the cells are allowed to become confluent.
Subcultivation Ratio: A subcultivation ratio of 1:20 to 1:40 is recommended
Medium Renewal: 2 to 3 times per week
Storage temperature: liquid nitrogen vapor phase | | Consigner: | UBIRATAN FABRES MACHADO
INSTITUTO DE CIENCIAS BIOMEDICAS- USP | | Reference: | 1064: Mandel JL, Pearson ML. Insulin stimulates myogenesis in a rat myoblast line. Nature 251: 618-620, 1974. PubMed: 4421831
22255: Richler C, Yaffe D. The in vitro cultivation and differentiation capacities of myogenic cell lines. Dev. Biol. 23: 1-22, 1970. PubMed: 5481965
22581: Yaffe D. Retention of differentiation potentialities during prolonged cultivation of myogenic cells. Proc. Natl. Acad. Sci. USA 61: 477-483, 1968. PubMed: 5245982
33164: Osawa H, et al. Identification and characterization of basal and cyclic AMP response elements in the promoter of the rat hexokinase II gene. J. Biol. Chem. 271: 17296-17303, 1996. PubMed: 8663388
33165: Osawa H, et al. Analysis of the signaling pathway involved in the regulation of hexokinase II gene transcription by insulin. J. Biol. Chem. 271: 16690-16694, 1996. PubMed: 8663315 | | Additional Info: | The L6 myogenic line was isolated originally by Yaffe from primary cultures of rat thigh muscle maintained for the first two passages in the presence of methyl cholanthrene. [22581]
L6 cells fuse in culture to form multinucleated myotubes and striated fibers. The extent of cell fusion declines with passage and the cells should be frozen at low passage and periodically recloned with selection for fusion competent cells.
Tested and found negative for ectromelia virus (mousepox). | | ATCC: | CRL-1458 | | Biosafety: | 1 |
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