| BCRJ: | 0127 |
| Cell Line: | K562-LUCENA |
| Tissue: | HEMATOPOIETIC |
| Organ: | |
| Cell Type: | MYELOBLAST |
| Morphology: | LYMPHOBLAST |
| Pathology: | |
| Scientific Name: | _HOMA SAPIENS |
| Vulgar Name: | HUMAN |
| Sex: | Female |
| Miscelaneous Info: | 54 YEAR OLD |
| Virus Succeptibility: | |
| Virus Resistance: | |
| Tumor Formation: | IN NUDE MICE |
| Products: | Glicoprotein - P ( MDR-1) |
| Dependency Isoenzymes: | |
| Culture Medium: | RPMI-1640 medium 90%, fetal bovine serum 10%
Subculture procedure: maintain cultures from 1x10E5 to 1x10E6 cells/mL.
Frequently it is necessary to add 60nM of Vincristine in culture medium to avoid
phenotype transformation. |
| Consigner: | Dr. Viviam M. Rumjaneck
Lab. Imunologia Tumoral
Depto Bioquímica Médica
ICB/UFRJ |
| Reference: | Ciência e Cultura, 46:63-69, 1994
Braz.J. Med. Biol. Res, 29:401-542, 1996 |
| Additional Info: | The K562-Lucena cell line was stablished from K562 cell line under pressure of
gradual vincristine supplement in culture medium. It express the P-Glicoprotein,
and has a Multi Drug Resistance (MDR) phenotype. Restricted distribution. Only to
be distributed with formal authorization of Dr. V. Rumjaneck. |
| ATCC: | |
| Biosafety: | 01 |
