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BCRJ:	0121
Cell Line:	J774 A.1
Tissue:	HEMATOPOIETIC
Organ:
Cell Type:	MONOCYTE
Morphology:	MACROPHAGE
Pathology:	SARCOMA
Scientific Name:	MUS MUSCULUS
Vulgar Name:	MOUSE
Sex:	Female
Miscelaneous Info:
Virus Succeptibility:	UNKNOWN
Virus Resistance:	UNKNOWN
Tumor Formation:
Products:	Interleukin-1; IL-1; Lisozim; NO Sintase; Complement Receptor; Immunoglobulin Receptor
Dependency Isoenzymes:	Lisozim
Culture Medium:	Dulbecco¦s Modified Eagle Medium (DMEM) suplemented with 10% of fetal calf serum. Cells must be scraped or disloged from tissue culture flask. Split ratio rom 1:3 to 1:5 is recommended.
Consigner:	Dr. Patrícia Dias Fernandes Depto Farmacologia Biofísica e Clínica ICB/CCS/UFRJ & Dr. Sonia Rosental Instituto de Biofísica Carlos Chagas Fo CCS/UFRJ
Reference:	Cancer Res, 37:546-550, 1997 J. Immunol, 114:898-905, 1975 Nature, 257:393-394, 1975 J. Exp. Med., 143:1528-1533, 1976
Additional Info:	This cell line was adpted in culture from female Balb/c tumor in 1978. LPS, PPD and Dextran Sulphate can inhibit cell growth. May be distributed for research applications. Not for commercial purposes.
ATCC:	TIB67
Biosafety:	01