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| BCRJ: | 0118 | | Cell Line: | IMR-90 | | Tissue: | | | Organ: | LUNG | | Cell Type: | FIBROBLAST | | Morphology: | FIBROBLAST | | Pathology: | NORMAL | | Scientific Name: | HOMO SAPIENS | | Vulgar Name: | HUMAN | | Sex: | FEMALE | | Miscelaneous Info: | | | Virus Succeptibility: | Human poliovirus 1, Human poliovirus 2, Human poliovirus 3, Varicella-Zoster, Vaccinia virus | | Virus Resistance: | | | Tumor Formation: | | | Products: | | | Dependency Isoenzymes: | | | Culture Medium: | The base medium for this cell line is Eagle's Minimum Essential Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: Every 3 to 4 days
Storage temperature: liquid nitrogen vapor phase | | Consigner: | LUIZ SOARES/ PRISCILA MENEZES
BOTICARIO | | Reference: | 22381: Nichols WW, et al. Characterization of a new human diploid cell strain, IMR-90. Science 196: 60-63, 1977. PubMed: 841339
32932: Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642
33041: Ostlund RE Jr., et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564 | | Additional Info: | The human diploid fibroblast strain IMR-90 was derived by W.W. Nichols and associates from the lungs of a 16-week female fetus. [22381]
The division potential, viral susceptibilities and other properties have been thoroughly studied such that the line may be considered as an alternate for WI-38 and other standard human lung cell strains.
The cells have been reported to be capable of attaining 58 population doublings before the onset of senescence. [22381]
Virus Susceptibility:
Human poliovirus 1
Human poliovirus 2
Human poliovirus 3
Varicella-Zoster
Herpes simplex virus 1
Herpes simplex virus 2
Vaccinia virus
Human herpesvirus 5
Vesicular stomatitis virus
DNA Profile (STR):
Amelogenin: X
CSF1PO: 11,13
D13S317: 11,13
D16S539: 10,13
D5S818: 12,13
D7S820: 9,12
THO1: 8,9.3
TPOX: 8,9
vWA: 16,19
Cytogenetic Analysis:
normal human female; diploid; stable | | ATCC: | CCL-186 | | Biosafety: | 1 |
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