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| BCRJ: | 0117 | | Cell Line: | IEC-6 | | Tissue: | EPITHELIUM | | Organ: | INTESTINE | | Cell Type: | EPITHELIAL | | Morphology: | EPITHELIAL | | Pathology: | NORMAL | | Scientific Name: | RATTUS NORVEGICUS | | Vulgar Name: | RAT | | Sex: | | | Miscelaneous Info: | | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | COLLAGEN; FIBRONECTIN | | Dependency Isoenzymes: | | | Culture Medium: | Dulbecco's MEM with 4.5 g/L glucose, 95%; fetal bovine serum 5%
and insulin at 0.1 U/mL. Subculture procedure: standard trypsinization
techniques. A split ratio of 1:3 to 1:6 is recommended. | | Consigner: | European Collection of Animal Cell Culture; through Dr. Wanderley
de Souza, Instituto de Biofisica, UFRJ, Rio de Janeiro. | | Reference: | J. Cell Biol. 80: 248-265, 1979. | | Additional Info: | These normal rat epithelial cells from the small intestine should
undergo at least 15 population doubling. IEC-6 cells synthesize
collagen and fibronectin, their growth is inhibited by cortisol,
and they possess cell surface antigens specific for intestinal
epithelial cells in vivo. | | ATCC: | CRL 1592 | | Biosafety: | 01 |
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