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BCRJ: | 0116 | Cell Line: | IEC-18 | Tissue: | EPITHELIUM | Organ: | INTESTINE | Cell Type: | EPITHELIAL | Morphology: | EPITHELIAL | Pathology: | NORMAL | Scientific Name: | RATUS NORVEGICUS | Vulgar Name: | RAT | Sex: | | Miscelaneous Info: | | Virus Succeptibility: | | Virus Resistance: | | Tumor Formation: | | Products: | | Dependency Isoenzymes: | | Culture Medium: | Dulbecco's MEM with 4.5 g/L glucose,95%; fetal bovine serum 5% and
insulin 0.1 U/mL. Subculture procedure: standard trypsinization
techniques. A split ratio of 1:3 to 1:6 is recommended. | Consigner: | Europen Collection of Animal Cell Culture; through Dr. Wanderley
de Souza, Instituto de Biofisica, UFRJ, Rio de Janeiro. | Reference: | J. Cell Biol. 80: 248-265, 1979;
J. Nat. cancer Inst. 67: 1353-1362. | Additional Info: | Normal rat epithelial cells from the ileum wich exhibittissue specific
surface antigens. Cells undergo at least 20 population doubling. | ATCC: | CRL 1589 | Biosafety: | 01 |
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