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BCRJ: | 0112 | Cell Line: | HTC | Tissue: | | Organ: | LIVER | Cell Type: | EPITHELIAL | Morphology: | EPITHELIAL | Pathology: | HEPATOMA | Scientific Name: | RATTUS NORVEGICUS | Vulgar Name: | RAT; BUFFALO STRAIN | Sex: | MALE | Miscelaneous Info: | | Virus Succeptibility: | | Virus Resistance: | | Tumor Formation: | | Products: | | Dependency Isoenzymes: | | Culture Medium: | Dulbecco's MEM 90%, fetal bovine serum 10%.
Subculture procedure: standard trypsinization techniques | Consigner: | Dr. Lydie Tichonicky, Institut de Pathologie Moleculaire, INSERM,
24 Rue du Faubourg St Jaccques, 75014, Paris, France. | Reference: | Thomson B. in Methods in Enzymology 58: 544-552, 1979;
Proc. Natl. Acad. Sci. USA 56: 295-, 1966. | Additional Info: | HTC (Hepatoma, Tissue Culture) cells were established from the
ascites of the rat hepatoma Morris 7288C. The original hepatoma
was induced by chemical carcinogem in male Buffalo strain inbred
rat. It was subsequently converted to the ascites form, and HTC
line was cultured directly from ascitic fluid. These cells are
useful in studyingboth liver and general cellular processes. They
have been specially used for studies of steroid hormonal effect
on enzyme induction and general metabolism, cholesterol synthesis
and growth control. | ATCC: | | Biosafety: | 01 |
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