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BCRJ: 0112
Cell Line: HTC
Tissue:
Organ: LIVER
Cell Type: EPITHELIAL
Morphology: EPITHELIAL
Pathology: HEPATOMA
Scientific Name: RATTUS NORVEGICUS
Vulgar Name: RAT; BUFFALO STRAIN
Sex: MALE
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:
Products:
Dependency Isoenzymes:
Culture Medium: Dulbecco's MEM 90%, fetal bovine serum 10%. Subculture procedure: standard trypsinization techniques
Consigner: Dr. Lydie Tichonicky, Institut de Pathologie Moleculaire, INSERM, 24 Rue du Faubourg St Jaccques, 75014, Paris, France.
Reference: Thomson B. in Methods in Enzymology 58: 544-552, 1979; Proc. Natl. Acad. Sci. USA 56: 295-, 1966.
Additional Info: HTC (Hepatoma, Tissue Culture) cells were established from the ascites of the rat hepatoma Morris 7288C. The original hepatoma was induced by chemical carcinogem in male Buffalo strain inbred rat. It was subsequently converted to the ascites form, and HTC line was cultured directly from ascitic fluid. These cells are useful in studyingboth liver and general cellular processes. They have been specially used for studies of steroid hormonal effect on enzyme induction and general metabolism, cholesterol synthesis and growth control.
ATCC:
Biosafety: 01


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