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BCRJ: 0111
Cell Line: HT-29
Tissue:
Organ: COLON
Cell Type: EPITHELIAL
Morphology: EPITHELIAL
Pathology: ADENOCARCINOMA
Scientific Name: HOMO SAPIENS
Vulgar Name: HUMAN; CAUCASIAN
Sex: FEMALE
Miscelaneous Info: 44 YEAR-OLD
Virus Succeptibility:
Virus Resistance:
Tumor Formation: IN STEROID TREATED HAMSTERS
Products:
Dependency Isoenzymes: Me2, 1; PGM3, 1-2; PGM1, 1-2; ES D, 1; AK1, 1; GLO-1, 1-2; G6PD, B.
Culture Medium: DMEM medium 90%, fetal bovine serum 10%. Subculture procedure: standart tripsinization techniques. A split ratio from 1:3 to 1:5 is recommended.
Consigner: Banco de Células do Rio de Janeiro
Reference: J. Clin. Pathol. 48(2): 143-6, 1995 Br. J. Cancer, 71(4): 744-52, 1995
Additional Info: The HT-29 cell was isolated from a primary tumor in 1964 by J.Fogh using the explant culture method and medium F12 with 15% fetal bovine serum. More recently, established cultures have been propagated in McCoyÆs 5a medium with the same serum suplement. May be distributed to scientific institutions; not to distributed for any commercial use.
ATCC: HTB38
Biosafety: 01


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