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BCRJ: | 0111 | Cell Line: | HT-29 | Tissue: | | Organ: | COLON | Cell Type: | EPITHELIAL | Morphology: | EPITHELIAL | Pathology: | ADENOCARCINOMA | Scientific Name: | HOMO SAPIENS | Vulgar Name: | HUMAN; CAUCASIAN | Sex: | FEMALE | Miscelaneous Info: | 44 YEAR-OLD | Virus Succeptibility: | | Virus Resistance: | | Tumor Formation: | IN STEROID TREATED HAMSTERS | Products: | | Dependency Isoenzymes: | Me2, 1; PGM3, 1-2; PGM1, 1-2; ES D, 1; AK1, 1; GLO-1, 1-2; G6PD, B. | Culture Medium: | DMEM medium 90%, fetal bovine serum 10%. Subculture procedure:
standart tripsinization techniques. A split ratio from 1:3 to 1:5 is recommended. | Consigner: | Banco de Células do Rio de Janeiro | Reference: | J. Clin. Pathol. 48(2): 143-6, 1995
Br. J. Cancer, 71(4): 744-52, 1995 | Additional Info: | The HT-29 cell was isolated from a primary tumor in 1964 by J.Fogh using the
explant culture method and medium F12 with 15% fetal bovine serum. More
recently, established cultures have been propagated in McCoyÆs 5a medium with
the same serum suplement. May be distributed to scientific institutions; not to
distributed for any commercial use. | ATCC: | HTB38 | Biosafety: | 01 |
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