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Results of Search
| BCRJ: | 0105 | | Cell Line: | HNK-1 [HNK1, Leu7] | | Tissue: | | | Organ: | SPLEEN | | Cell Type: | HYBRIDOMA | | Morphology: | LYMPHOBLAST | | Pathology: | | | Scientific Name: | MUS MUSCULUS | | Vulgar Name: | MOUSE | | Sex: | | | Miscelaneous Info: | | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | immunoglobulin; monoclonal antibody; against human natural killer (NK) cells and antigen dependent killer (K) cells (CD57) | | Dependency Isoenzymes: | | | Culture Medium: | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.02 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. | | Consigner: | ANDREA GONÇALVES TRENTIN
UFSC- CCB | | Reference: | 928: Abo T, Balch CM. A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). J. Immunol. 127: 1024-1029, 1981. PubMed: 6790607
929: Abo T, et al. Postnatal expansion of the natural killer and killer cell population in humans identified by the monoclonal HNK-1 antibody. J. Exp. Med. 155: 321-326, 1982. PubMed: 7054358
22915: Schuller-Petrovic S, et al. A shared antigenic determinant between natural killer cells and nervous tissue. Nature 306: 179-181, 1983. PubMed: 6196639
22916: McGarry RC, et al. Recognition of myelin-associated glycoprotein by the monoclonal antibody HNK-1. Nature 306: 376-378, 1983. PubMed: 6196641
23000: Vincent M, Thiery JP. A cell surface marker for neural crest and placodal cells: further evolution in peripheral and central nervous system. Dev. Biol. 103: 468-481, 1984. PubMed: 6202575
23132: McBurney MW, et al. Differentiation and maturation of embryonal carcinoma-derived neurons in cell culture. J. Neurosci. 8: 1063-1073, 1988. PubMed: 2894413
23138: Tucker GC, et al. Identical reactivity of monoclonal antibodies HNK-1 and NC-1: conservation in vertebrates on cells derived from the neural primordium and on some leukocytes. Cell Differ. 14: 223-230, 1984. PubMed: 6207939 | | Additional Info: | | | ATCC: | TIB-200 | | Biosafety: | 1 |
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