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| BCRJ: | 0104 | | Cell Line: | HL60 | | Tissue: | HEMATOPOIETIC | | Organ: | PERIPHERAL BLOOD | | Cell Type: | MYELOBLAST | | Morphology: | LYMPHOBLAST | | Pathology: | LEUKEMIA | | Scientific Name: | HOMO SAPIENS | | Vulgar Name: | HUMAN; CAUCASIAN | | Sex: | FEMALE | | Miscelaneous Info: | 36 YEARS OLD | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | | | Dependency Isoenzymes: | | | Culture Medium: | RPMI-1640 medium 80%, fetal bovine serum 20%.
Subculture procedure: maintain cultures from 5x10E4 to 5x10E5 cells/mL. | | Consigner: | Cristiano Ponte - CEFET-Q - Unidade Maracanã - Biotecnologia - Cultura de Células e Tecidos Animais - Rua Senador Furtado 121 Maracanã - Rio de Janeiro/RJ CEP 20.270-021 55 21 3978-5902 | | Reference: | Nature 270: 347-49, 1977;
Blood 54: 713-33, 1979. | | Additional Info: | Peripheral blood leukocytes were obtained by leukophoresis from
a 36 year-old Caucasian female with acute promyelocytic leukemia.
The predominant cell population consists of neutrophilic
promyelocytes with prominent nucleo/cytoplasmatic asynchrony. Up to
10% of the cultured cells spontaneously differentiate beyond the
promyelocytic stages and the proportion is enhanced by polar-planar
compounds, such as DMSO. The HL60 cells lack specific markers for
lymphoid cells, but expresses surface receptors for Fc fragment and
complement. They exhibit phagacytic activity and responsiveness to
chemotatic stimuli. | | ATCC: | CCL-240 | | Biosafety: | 01 |
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