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BCRJ:	0102
Cell Line:	Hepa-1c1c7
Tissue:
Organ:	LIVER
Cell Type:	B LYMPHOCYTE
Morphology:	EPITHELIAL
Pathology:	HEPATOMA
Scientific Name:	MUS MUSCULUS
Vulgar Name:	MOUSE
Sex:
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:
Products:	cytochrome P450IA1
Dependency Isoenzymes:
Culture Medium:	Alpha minimum essential medium without nucleosides, 90%; fetal bovine serum, 10% Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: Every 2 to 3 days Doubling Time: 18 hrs Storage temperature: liquid nitrogen vapor phase
Consigner:	CRISTIANE PIENNA SOARES FACULDADE DE CIENCIAS FARMACEUTICA - SP
Reference:	22320: Bernhard HP, et al. Expression of liver phenotypes in cultured mouse hepatoma cells: synthesis and secretion of serum albumin. Dev. Biol. 35: 83-96, 1973. PubMed: 4362668 22342: Hankinson O, et al. Genetic and molecular analysis of the Ah receptor and of Cyp1a1 gene expression. Biochimie 73: 61-66, 1991. PubMed: 1851644 22593: Hankinson O. Single-step selection of clones of a mouse hepatoma line deficient in aryl hydrocarbon hydroxylase. Proc. Natl. Acad. Sci. USA 76: 373-376, 1979. PubMed: 106390
Additional Info:	This is a derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse. The cells are highly inducible for cytochrome P450IA1. This and mutant derivatives of it are useful for studying the aryl hydrocarbon (Ah) receptor and P450IA1 regulation.
ATCC:	CRL-2026
Biosafety:	1