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BCRJ: 0101
Cell Line: HEP-2
Tissue: EPITHELIUM
Organ: LARYNIX
Cell Type: EPITHELIAL
Morphology: EPITHELIAL
Pathology: CARCINOMA
Scientific Name: HOMO SAPIENS
Vulgar Name: HUMAN
Sex: MALE
Miscelaneous Info: 56 YEARS OLD, HELA MARKERS
Virus Succeptibility: POLIOVIRUS 1; ADENOVIRUS 3; VESICULAR STOMATITIS (
Virus Resistance:
Tumor Formation:
Products:
Dependency Isoenzymes: G6PD-A
Culture Medium: Dulbecco's MEM 90%, fetal bovine serum 10%. Subculture procedure: standard trypsinization techniques. A split ratio 1:6 is recommended.
Consigner: ATCC; through the Departamento de Virologia, Instituto de Microbiologia, UFRJ.
Reference: Cancer Res. 15: 598-, 1955; Texas Rep. Biol. Med. 15: 588-, 1957; Ann. N. Y. Accad. Sci. 76: 497-,1958.
Additional Info: The HEp-2 cell line was established from epidermoid carcinoma of larynx. It was extensively used for growth of arboviruses. Hep-2 cells have HeLa chromossome markers and G6PD type A, not expected type B. This line was probably contaminated with HeLa cells. These cells are positive for keratin by immunoperoxidase staining. Biosafety:2 [Cells contain Papovavirus]
ATCC: CCL-23
Biosafety: 02


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