| BCRJ: | 0098 |
| Cell Line: | H9c2 (2-1) |
| Tissue: | MYOCARDIUM |
| Organ: | HEART |
| Cell Type: | MYOBLAST |
| Morphology: | MYOBLASTIC-LIKE |
| Pathology: | |
| Scientific Name: | RATTUS NORVEGICUS |
| Vulgar Name: | RAT |
| Sex: | |
| Miscelaneous Info: | Age: embryo |
| Virus Succeptibility: | |
| Virus Resistance: | |
| Tumor Formation: | |
| Products: | Myokinase; creatine phosphokinase; myosin. |
| Dependency Isoenzymes: | |
| Culture Medium: | Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; fetal bovine serum, 10%.
Subculture procedure: standard trypsinization techniques.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium renewal: 2 to 3 times per week |
| Consigner: | Sara Terezinha Olalla Saad - UNICAMP - Hemocentro II - Clínica Médica - Biologia Molecular - Rua Carlos Chagas 480 - Cidade Universitária - Campinas - São Paulo - Brasil - 13083-970 - Fone: 55 19 37888734 |
| Reference: | 1062: Kimes BW , Brandt BL . Properties of a clonal muscle cell line from rat heart. Exp. Cell Res. 98: 367-381, 1976. PubMed: 943302
32970: Levy AP , et al. Post-transcriptional regulation of vascular endothelial growth factor by hypoxia. J. Biol. Chem. 271: 2746-2753, 1996. PubMed: 8576250 |
| Additional Info: | H9c2(2-1) is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue by B. Kimes and B. Brandt and exhibits many of the properties of skeletal muscle.
Myoblastic cells in this line will fuse to form multinucleated myotubes and respond to acetylcholine stimulation.
Fusion occurs faster if the serum concentration in the medium is reduced to one percent.
The myoblastic population will become depleted rapidly if the cultures are allowed to become confluent.
To prevent loss of myoblastic cells, cultures should be subcultured before they become confluent, and the line should be recloned periodically with selection for myoblastic cells. |
| ATCC: | CRL-1446 |
| Biosafety: | 01 |
