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Results of Search
| BCRJ: | 0094 | | Cell Line: | GRX | | Tissue: | CONNECTIVE | | Organ: | LIVER | | Cell Type: | FIBROBLAST | | Morphology: | FIBROBLAST | | Pathology: | SCHISTOMIASIS | | Scientific Name: | MUS MUSCULUS | | Vulgar Name: | MOUSE; C3H/HE | | Sex: | | | Miscelaneous Info: | | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | Collagen; fibronectin; laminin; heparan sulfate; chondroitin sulfate | | Dependency Isoenzymes: | | | Culture Medium: | Dulbecco's MEM 95%, fetal bovine serum 5%.
Subculture procedure: standard trypsinization techniques;
a split ratio of 1:4 to 1:6 is recommended. | | Consigner: | Dr. Radovan Brojevic, Departamento de Bioquimica, Inst. de Química,
UFRJ, Rio de Janeiro. | | Reference: | In Vitro Cell. Develop. Biol. 21: 382-390, 1985;
Biochim. Biophys. Acta 1011: 1-5, 1989;
In Vitro cell. Develop. Biol. 26: 361-368, 1990. | | Additional Info: | GRX cell line was established from primary cultures obtained by
spontaneous migration of cells from granulomas induced in liver of
C3H/Hej mice infected with Schistosoma mansoni. These cells display
myofibroblast characteristics,and are considered to represent murine
liver cconnective tissue. They can be induced in vitro to display a
fat-storing phenotype corresponding to Ito cells by treatment with
retinol, insulin, and indomethacin. GRX cells produce viral particles of
the retrovirus type, are anchorage dependent and have low contact
inhibition. Several clones are available, contact Rio de Janeiro Cell Bank
for further informations. | | ATCC: | | | Biosafety: | 01 |
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